Authors : Sapana B. Baviskar, Shanti R. Patil, Sneha R. Patil and Rajesh D. Deotale
Page Nos : 435-439
Description :
Thirteen genotypes belonging to five species Brassica rapa, Brassica juncea, Brassica carinata, Brassica napus and Eureca sativa were evaluated for molecular traits to study the diversity among the genotypes and to identify the desirable and potential parents for mustard breeding programme during the year 2008-09 at College of Agriculture Nagpur. The molecular markers (RAPD) was found to show polymorphism. The genomic DNA from the mustard genotypes when amplified using eighteen random primers gave 279 scorable amplified product. The maximum number of bands were generated by the primer E07, E09 and IOP700 (19). The similarity index between the drought tolerant genotypes RTM-314 and T-27 belonging to Eureca sativa with the cultivated genotypes Pusa Bold and ACN-9 belonging to Brassica juncea were found to be 0.21 to 0.30 which indicates a high level of dissimilarity and diversity among these two species. Drought tolerant genotype RTM-314 and T-27 deviated from all other genotypes on the basis of molecular markers. This study, lead to conclusion that the cultivated genotype ACN-9 and Pusa Bold of Brassica juncea can be further improved by crossing with RTM-314 and T-27 (Eureca sativa) for drought tolerance.
Keywords: Mustard genotypes, molecular markers, RAPD, diversity
