Authors : S. S. Sheikh, V. P. Dakhane, A. D. Chaudhary
Page Nos : 101-108
Description :
Abstract:
Bacopa monnieri (L.) Pennell, is an annual herb of the family scrophulariaceae. In vitro callus induction protocol through young leaf, mature leaf, nodal and internodal segment culture was achieved when Murashige and Skoog medium supplemented with different combinations of phytoharmones. Of all the explants mature and young leaf were found to be best explant for callus induction. The MS-medium supplemented with 0.8mg/l 2,4-D and 0.3 mg/l kinetin was the best medium for callus induction in mature leaf. The very simple and effective protocol developed can be used for the large scale cultivation of this very important medicinal herb.
Keywords:- Bacopa monnieri, Brahmi, Callus induction, Tissue Culture, In vitro callus Culture.
