Authors : Ashwin Butle & Mandakini Patil
Page Nos : 622- 634
Description :
Hepatic tissue damage causes impairment in liver function which has harmful effect on human health. Management of liver diseases is still a challenge in developing world. The present study has been carried out to evaluate in vitro and in vivo hepatoprotective activity of triterpenoids isolated from Zizyphus mauritiana leaves. Zizyphus mauritiana ethyl acetate (ZMEA) extract was further purified on silica gel named as Z. mauritiana triterpenoids (ZMT) and tested for its hepatoprotective potential in vitro by using HepG2 cell line and in vivo by using Wistar albino rats. GC-MS chromatogram shows that ZMT contains triterpenoids. In vitro MTT assay depicts the protective activity of ZMT on CCl4 intoxicated HepG2 cells. The results of biochemical parameter showed that ZMT has an efficacy to restore the liver function in vivo. The restoration property of hepatic cells by ZMT shows comparable results with standard sylimarin, which was also observed in liver histological examination of Wistar albino rat. Results of this study strongly demonstrate ZMT has been found to have promising hepatoprotective potential.
