Authors : Prajapati N.B and Raval A.A
Page Nos : 65-77
Description :
The present study aimed to synthesize zinc nanoparticles (ZnNPs) through a biogenic approach using the bacterial isolate
KNH3 obtained from agricultural soil. The synthesized ZnNPs were characterized using UV–Visible spectroscopy,
Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR). UV–Visible spectral analysis
revealed a characteristic Surface Plasmon Resonance (SPR) peak at 390 nm, confirming the formation of ZnNPs. SEM
analysis demonstrated that the nanoparticles were predominantly spherical with particle sizes ranging from 62 to 89
nm. FTIR spectra indicated the presence of various biomolecules functioning as reducing, capping, and stabilizing agents
during nanoparticle synthesis. The antimicrobial potential of the biosynthesized ZnNPs was evaluated against
phytopathogenic microorganisms, including Agrobacterium tumefaciens, Arthrobacter sp., Rhizoctonia solani, and
Sclerotium rolfsii. The ZnNPs exhibited significant antimicrobial activity against all tested pathogens, highlighting their
potential application in plant disease management. Furthermore, the influence of biosynthesized ZnNPs on the growth
of Solanum lycopersicum seedlings was investigated. Treatment with ZnNPs significantly enhanced root length, hypocotyl
length, and fresh biomass compared to untreated controls. These findings demonstrate that biogenically synthesized
ZnNPs possess promising antimicrobial properties and can effectively promote seedling growth, suggesting their potential
utility in sustainable agricultural applications.
