Authors : Swati Gaikwad , Sujata Bhargava, Vaibhav Gurve , Pratiksha Getme, Roshan Yelne, Raviraj Udasi
Page Nos : 141-147
Description :
In vitro banana propagation, especially meristem tip culture, has played a key role for obtaining a large number of virus free and homogenous planting material in Musa spp. The experiment was carried out in Botany section, Bajaj College of Agriculture, Wardha, Maharashtra India. The plantlet was regenerated using different concentration of BAP with MS medium. Maximum shoot multiplication observed at MS Medium + 3 mg l-1 BAP + 200 mg l -1 cefotaxime + 50 mg l -1 Bavistin. In vitro banana propagation, especially meristem tip culture, has played a key role for obtaining a large number of virus free and homogeneous planting material in Musa spp. In our previous study, we have standardized complete protocol for the banana meristem tip culture. The experiment was carried out in Botany Section, Bajaj College of Agriculture, Wardha (Affiliated to Dr. PDKV Akola, India). The plantlet was regenerated using different concentration of BAP with MS medium. Maximum shoot multiplication observed at 3 mg l-1 BAP + MS medium + 200 mg -1 cefotaxime + 50 mg l-1 Bavistin. Tissue culture raised plantlet, its mother culture and mother plant tested against the four different viruses mostly found in banana plant. The virus indexing of banana leaf sample of Grand naine variety were carried out for four viruses i.e. cucumber mosaic virus (CMV), Banana bract mosaic virus (BBrMV) by DAS-ELISA and banana steak Mysore virus (BSMYV), bunchy top virus (BBTV) by Duplex PCR. None of the virus were detected in any of the sample. Additionally, genetic fidelity analysis was also carried out with ISSR primers and no polymorphism was detected.
