Authors : Dipak V. Kumbhar, B. K. Avchar, Amol S. Bhosale
Page Nos : 8-14
Description :
Momordica cymbalaria Fenzal., a medicinally important Cucurbitaceae species, is traditionally used for its anti-diabetic, anti-inflammatory, and nutritional properties. despite its cultivation, it faces challenges such as low germination rates and limited genetic diversity. Induced mutagenesis using Ethyl Methane Sulphonate (EMS) offers a potential solution by generating genetic variability for improved traits. This study investigates the dose-dependent effects of EMS on tuber regeneration, morphological traits, and genetic diversity in Momordica cymbalaria. Tubers were treated with EMS concentrations ranging from 10 mM to 50 mM to assess their impact on regermination, survival, and growth parameters. Results indicate that moderate EMS doses enhance callus formation and shoot regeneration, whereas higher concentrations reduce survival rates. Significant variations in morphological traits, including plant height, leaf structure, and tuber morphology, were observed among mutants. Results revealed a dose-dependent decline in regermination and survival rates, with moderate concentrations (10–30 mM) inducing beneficial mutations while maintaining viability, whereas higher concentrations (40–50 mM) severely impaired plant development. The study identifies optimal EMS concentrations for balancing mutation induction and plant viability, offering a promising approach for conservation and mutation breeding of this rare medicinal plant. These findings contribute to the sustainable utilization of Momordica cymbalaria in agriculture and traditional medicine.
