Authors : S. A. Karande, A. G. Tekale, A. M. Kadam, B. K. Chavan and V. D. Darandale
Page Nos : 46-54
Description :
Chilli (Capsicum annuum L.), is an important solanaceous vegetable-cum-spice crop.Chilli requires hot and humid conditions for growth and development and its cultivation is mainly confined to the tropical regions of the world. Chilli crop is suffered from many fungal, bacterial and viral diseases. Therefore, present in vitro study was conducted to assess Efficacy of bioagents against Sclerotium rolfsii of Chilli. Infected chilli plants showing typical root rot symptoms were collected from the fields and isolated on PDA. Pathogenicity of the test fungi were proved by sick soil (S. rolfsii) method in earthen pots, under screen house conditions. Nine bioagents were evaluated in vitro against the test fungi S. rolfsii, by applying dual culture technique and using potato dextrose agar (PDA) as basal culture medium. Observations on radial mycelial / growth colony diameter (mm) of the test fungi at an interval of 24 hrs of incubation were recorded and continued up to seven days or till the untreated PDA plates were covered fully with mycelial growth of the test fungi. All the nine bioagents evaluated in vitro, were found effective against S. rolfsii and significantly inhibited its mycelial growth over untreated control. However, highest mycelial growth inhibition recorded by T. asperellum (49.50 mm and 45.00 %), Aspergillus niger (51.50 mm and 42.77%), T. hamatum (55.00 mm and 38.88 %), T. koningii (55.00 mm and 38.88 %), Verticillium lacani (70.00 mm and 22.22 %), Metarhizium anisopliae (80.50 mm and 10.55 %), Pseudomonas fluorescens (88.50 mm and 1.66 %) and Bacillus subtilis (90.00 mm and 0.00 %).
