Authors : Bharti Ghude Wadekar
Page Nos : 156-166
Description :
The bacteriocin B25 produced by Marine Lactobacillus pentosus B25. Purification was carried by ammonium sulphate precipitation, gel filtration chromatography. Gel filtration chromatography provides 3.04 fold increase in specific activity (553.84 AU/mg). The total production found 27 ml that contain 7.8 mg/ml bacteriocin concentration. This bacteriocin B25 was found to be stable up to 1100C at 20 min, so it is heat stable protein and active over a wide pH range of 4.0-10.0. It was proteinases nature, because their activity inhibited by proteinase K enzyme. It remained active in Triton X-100, Tween-80, Tween 20 and SDS but lost its activity when treated by urea and EDTA. Molecular weight of bacteriocin estimated by Glycine SDS-PAGE and found to be <3.5kDa.
This study describes the production, purification and characterization of bacteriocin B25.
