Authors : DVNS Suresh* and R S Bagade
Page Nos : 111-120
Description :
Although much of the aspects of its biology have been well studied, there is a lack of knowledge on the reproductive endocrinology of this Indian major carp. Labeo rohita is the most cultivable and relished fish of India which needs an attention on its high yield production with healthy and robust fish and an exclusive knowledge about its reproductive regulations and the steroidal involvements. The serum and tissue samples were subjected to sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS-PAGE) for the separation of proteins to isolate the SHBG in the gel and then transferred to a poly vinyledene difluride (PVDF) membrane. The transferred protein band can be analyzed for the immuno confirmation of SHBG with help of anti-SHBG (DSL, Texas, USA). With increased serum graded samples, immuno-confirmation was positively observed in incremental reactions against anti-SHBG. Although albumin has not been found in fish, proteins with some of the characteristics of albumin are present. Interference of high concentrations of vitellogenins, however, may generate small changes in kDa of SHBG. The binding characteristics of SHBG remain unchanged at these reproductive stages. The possible molecular weight of the Labeo rohita SHBG (lrSHBG) is approximately 30254 Da. There is a poor evolutionary conservation of SHBG among species of same class.
