Authors : A.R. Tuwar, S.D. Kadlag , R.G. Khose
Page Nos : 198-204
Description :
Antifungal activities of leaf extracts of Sphagneticola calendulacea (L.) Pruski of Asteraceae family were
investigated by using agar well diffusion method. Fungi viz. Aspergillus Niger, Aspergillus flavus,
Fusarium oxysporum and Rhizophus stolanifer were cultured on PDA agar medium separately in petri
plates of 11cm diameter. Wells of 10mm diameter were prepared in the agar medium with cork-borer
Fresh leaf extracts of 10, 20, 30 and 40% were prepared, sterilized and used. Sterile distilled water was
added in the central well and treated as control. Plates were incubated art 370C. Inhibition zones were
measured after 5 days. Well agar diffusion method: Leaf extract of 10%, 20%, 30% &40% concentration
added in separate well. Central well was added with water was treated as a control. Extract inhibited
the fungus growth around the wells. The inhibited zones around the wells were measured in millimetre.
Readings were recorded in tabular form. Photographs were taken by using digital camera.
