Authors : Mundeep G. Awaley
Page Nos : 26-35
Description :
The plants of Cajanus cajan were grown in plots from seeds obtained from Krishi Vigyan Kendra, Gondia. The well developed seeds of same age will be selected and will be subjected to gamma irradiation doses. Prior to the mutagenic treatments, all genotypes were grown for one generation to ensure their homozygosity. The well developed seeds of same age were selected and subjected to gamma irradiation doses of 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100 and 1200 Gy (Gray) (40 seeds for each treatment in four genotypes). Gamma irradiation will be carried out at at room temperature (22- 25oC) in a Cobalt 60 gamma cell-220 of 381.43 curie strength delivering 29kR/hr at the time of irradiation. For EMS treatment, the seeds were presoaked in sterile distilled water for 6 hours and then treated with different concentrations of EMS (10, 20, 30 and 40mM) for 8 hours. The mutagen treated and control (untreated) seeds were sown in the field in randomized block design (RDB), with three replications. The seeds of all individual M, plants were harvested separately and they were sown in the field during the next Kharif to rise M1 generation.
