Authors : K.N. Sathawane
Page Nos : 193-196
Description :
Induced mutagenesis is a useful tool and being used widely to create variability in plants. In the present study, three mutagens, ethyl methane sulphonate (EMS), sodium azide (SA) and gamma rays were used to create morphological variability in Brassica juncea cv Varuna. Dry and water pre-soaked seeds were used. EMS and SA treatment with dry seeds were of 12h duration whereas for 12 and 18h water pre-soaked, the treatment duration was 6h. The seeds were also exposed to 10, 20, 30 and 40kR gamma rays. In M1 generation, on maturity, M1 plants were harvested on plant wise basis. M2 generation was raised on M1 plant seeds. In M2 generation, EMS, SA and Gamma rays induced Appressed Pod mutation in variable frequency. Gamma rays induced higher frequency of compared to EMS and SA and breed true for M3 and M4 generations. Appressed pod mutants were characterised for their morphological and yields characters and screened out for crude fibre, crude protein, glucosinolate and Appressed Pod mutation erucic acid contents
