Authors : Sukeshini Deogade and P.N. Nasare
Page Nos : 188-192
Description :
Mitotic cell division was observed in root tips of varieties of Allium sativum L. i,e Agrifound white and Yamuna safed fixed in Carnoy’s fixative (Glacial acetic acid : absolute alcohol, 1:3) at different intervals of time, i.e 8.00-8.30am, 8.30-9.00am, 9.00-9.30am, 9.30-10.00am, 10.00-10.30am, 10.30-11.00am. The number of semipermanent squash preparations were observed for the study of mitotic activity. For each time 20 slides were observed to scored prophase, metaphase, anaphase and telophase. In the varieties of Agrifound white, maximum metaphases (9.10%) and anaphases (5.15%) observed at the time 9.00-9.30am, and in Yamun safed, maximum metaphases (10.10%) and anaphases (7.30%) observed at the time 9.30-10.00am respectively. The purpose of present study is to know the mitotic index and phase index in Allium sativum L. varieties (Agrifound white and Yamuna safed) for proper study of the chromosomes for karyotype analysis.
Keywords: Allium sativum L., Mitotic cell division, Mitotic index and Phase index.
