Authors : T. Srinivas, S. Indira and Bhadraiah
Page Nos : 176-78
Description :
The pathogen M.phaseolina was successfully isolated from the basal portion of the charcoal rot of infected Sorghum stem collected from the fields of National Research Centre for Sorghum (NRCS) Rajendranagar, Hydersbad. In this present study small bits of sclerotia bearing strands were surface sterilized by immersing in 0.1 percent mercury chloride for two minutes. The surface sterilized strands washed in three changes of sterile distilled water. They were planted on PDA under aseptic conditions and incubated at 25C in SEW,BOD incubator. After 4days of incubation the fungus was transferred on a fresh PDA plate and was purified through single sclerotia isolation purified culture of M.phaseolina was maintained on PDA slants at room temperature(26-28C)..
Keywords: Sclerotia, aseptic and Charcoal rot
