Authors : Patil S.R., Kuchanwar O.D., Deotale R.D., Jiotode D.J., Kalamkar V . B. and Rathod A .
Page Nos : 33-37
Description :
Colchicine at 0.0%, 0.5%, 1.0%, 1.5%, 2.0% and 2.5% was used for induction of variation
through polyploidy in marigold by two methods
viz.
seed treatment and root treatment during 2016.
Significant variation was observed between seed treatment method and
root treatment method for all the
traits studied. In seed treatment method colchiploid plants survived in all the concentration, but in root
treatment method colchiploid plants survived only upto 1.5% concentration. The plants were not able to
withstand th
e higher concentration of colchicines when roots were treated. The traits related to stomata,
guard cell and pollen grain served as useful indicator for induction of variation through polyploidy.
Frequency of stomata and pollen grain decreased with the in
crease in concentration of colchicines. On
the contrary the size of stomata and guard cell in terms of length and breadth and pollen grain size on
terms of diameter increased with increase in concentration. In addition treated plants were having larger
and
thick leaves with deep green pigmentation as compared to the untreated control. Maximum number
of types of variation were recorded in 0.5% colchicine in both seed and root treatment method. Seed
treatment method was found to be more efficient than root tr
eatment method in inducing variation
through polyploidy. Seeds soaked in colchicine solution at 0.5% concentration for 12 hrs was found most
effective in inducing large number of variants types. The variant plants scored on the basis of stomatal,
guard cel
l and pollen grain traits were labeled and harvested separately for further confirmation and
evaluation.
