Authors : Shilpa S.Burele , Hemalata Karkar and Vishakha Ganvir
Page Nos : 24-26
Description :
Gram negative hospital acquired infection are a major problem since last two decades Acinetobacter baumanii has emerged as a highly trouble pathogen for many institutions globally. As a consequence of its immense ability to acquired antibiotic drug resistance determinant .It has justifiably been propelled to the fore front of scientific attention. Rapid spread of multi drug resistant isolates causing infection. Several typing method have been described for Acinetobacter to establish a correlation between various epidemic strains and their source and mode of transmission some typing systems of Acinetobacter are profile of Acinetobacter are profile of biochemical tests, carbon source growth assays DNA-DNA hybridization, antibiotic susceptibility pattern (antibiograms ), protein eletrophoretic patterns and electrophoretic mobilities of cellular enzymes. The most popular rapid and simple method is plasmid profile strains with similar plasmid profiles have been further analysis by restrictions endonuclease digestion of plasmid DNA to generate plasmid finger print .for typing of Acinetobacter PCR based methods were used. Acinetobacter isolates are also identified to genomic species level by technique called amplified ribosomal DNA restriction analysis (ARDRA). Although many of typing systemic are based on the last taxonomic development biotyping serotyping, antibiotic typing enzyme, electro-eretic typing and plasmid profile ribotyping. The treatment of multidrug resistant Acinetobacter baumanii is a serious therapeutic problem due to the limited penetration of antibiotic.
Keywords: Acinetobacter isolates, Antibiotic Ampicillin, Resistant to Antibiotic Ampicillin.
