Authors : A. Pandey and S. K. Katiyar
Page Nos : 214-218
Description :
Abstract:
The success of the drug discovery process is often a function of the diversity of chemotypes examined. Natural products screening represents a potential source of organic chemicals of unparallel diversity. A medicinal herb can be compared with a chemical factory due to presence of number of chemical constituents like alkaloids, glycosides, saponins, resins, oleoresins, sesquiterpene lactones and oils (essential and fixed). In the present study we analyzed and quantified the saponin from musli (Chlorophytum) respectively for identification of best quality genotype. In order to determine the quantity of lead compound i.e. saponin in different genotypes, HPLC based protocol was standardized. Saponin extracted by 85% ethanol. Extracted material purified through column chromatography. After identification of saponin fraction by thin layer chromatography, extracted and purified sample subjected to HPLC for the estimation of saponin.
