Authors : J. B. Chawla
Page Nos : 324-334
Description :
Soils from various agricultural fields having pesticides exposure since several years were used for the isolation of monochlorophenol degrading bacteria. Two strains designated as strain A2 and strain J3 were isolated. Strain A2 was able to mineralize 2-, 3- as well as 4-chlorophenol.The rates of degradation of the three isomers were in the following order: 4-chlorophenol (4-CP) >3-chlorophenol(3-CP)>2-chlorophenol (2-CP).The maximum degradable concentrations of 4-CP,3-CP and 2-CP were 900,800 and <300µM respectively. Strain J3 was able to mineralize 2-CP only and could not mineralize the 3-CP or 4-CP. The maximum degradable concentration of 2-CP was 2300µM. On the basis of various morphological, cultural, biochemical and 16 S rRNA gene sequence analysis the strain A2 was identified as a member of Rhodococcus genus (most probably Rhodococcus coprophilus) while J3 was identified as a member of Achromo bacter genus (most probably Achromobacter xylosoxidans sub-sp. denitrificans).
