Authors : K. V. Dubey, P. N. Charde and G. L. Bhoosreddy
Page Nos : 318-323
Description :
Low yields biosurfactant are major factor for its popularity. Therefore, strain improvement by extensive application of mutation and selection through exposure of organism to mutagen for high yield is an appropriate measure. In the present study, mutation screening technique was developed as an important tool for screening of biosurfactant hyper-producing mutants. UV- irradiation was selected as a mutagen, the dose of UV irradiation was standardized and the process of mutant screening technique was developed using curd whey as a principal media component replacing the costly synthetic medium. Pseudomonas aeruginosa strain PP2 was exposed at to different doses of UV irradiation for standardization of dose and irradiated cells were plated on whey amended Cetyltrimethylammonium bromide (CTAB) + Methylene blue for the characterization of mutant with enhanced biosurfactant productivities. This screening technique is applicable for both standardization of mutagen dose and isolation of biosurfactant hyper- producing mutants on the basis of zone size of the halos that developed around the colonydue to the complexation ofanionic rhamnolipid with cationic Methylene blue and CTAB. The yields of biosurfactant produced by mutant strains were improved in the range of 22-60% as compared to wild strain.
