Authors : Jayashree B. Tirpude
Page Nos : 227-235
Description :
The polymerase chain reaction and its expanding numbers of modifications have become a mainstay in diagnostic and research medicine. The technique allows amplification of nucleic acid sequences both for the purposes of disease and pathogen detection and also for the preparation of hybridization probes and sequencing templates. PCR mimics the in vivo process of DNA replication with a sensitivity which enables detection of a single target sequence in 106 genomes. The principles of standard PCR, together with its more widely-used variations, are reviewed and their main applications outlined.
