Authors : M. N. Bhajbhuje
Page Nos : 214-220
Description :
Fungal metabolites are known biconcave organic compounds produced by diverse group of fungal organisms during growth and metabolism. Metabolites secreted in nutrient medium at different growth intervals by Alternaria alternate (Fr.) Keissler, a serious causal pathogen of leaf spot of Spinacia oleracea L were isolated, confirmed their chemical nature and evaluated for their cytological effects on metabolically active cells of meristematic zone of roots. An increase in per cent seed germination without any abnormalities over control was recorded with seeds receiving five days old metabolites treatment. Seed germination rate declined while percent abnormalities increased with metabolites of longer duration. Meristematic cells from root of treated seeds were reported to multiply abnormally due to appearance of many abnormalities like Anaphase Bridge, laggards, diagonal metaphase and mis-orientation of mitotic spindles. Phytochemicals tests and UV absorption spectrum for isolated toxins confirmed its phenolic nature.
