Authors : Khandare S. S. and Patil S. D.
Page Nos : 126-130
Description :
Idli batter samples were collected from market. Samples were properly diluted and were grown on MRS agar at 370 C for 2 to 3 days. Depending on colony characteristics, ten random colonies were selected and grown in MRS broth. The antibacterial activity of cell free supernatant of ten isolates against the food borne pathogens S. aureus and spoilage organism P. aeruginosa isolated from meat was studied. On the basis of zone of inhibition the highest potential bacteriocinogenic LAB-A, LAB-B and LAB-C were selected.Antibacterial activity of LAB isolates were compared with the indicator organism L. acidophilus MTCC 10307 obtained from MTCC Chandigarh.LAB-A, LAB-B and LAB-C showed 26.31, 15.78 and 26.31% increased bacteriocin production respectively tested against S.aureus and 10, 20 and 20% respectively tested against P. aeruginosathan L. acidophilus MTCC 10307. All LAB isolates exhibited antagonistic effect with highest spectrum of activity in LAB-A, LAB-B and LAB-C.
