Authors : Itankar P. R., Tajne M. R., Nagulwar D. G., Meshram S.
Page Nos : 470-477
Description :
In the present study, an attempt was made to the study included standardization of Rasnadi Guggul formulations by developing a simple, precise and accurate High Performance Thin Layer Chromatography (HPTLC) method using oleic acid and linoleic acid as chemical markers. Chromatographic analysis was performed on silica gel 60 F254 precoated TLC plates using Toulene: Ethyl acetate: Methanol: Acetic acid (9:1:0.2:0.5 v/v/v/v) for oleic acid and Toulene: Ethyl acetate: Formic acid (9:1:0.3 v/v/v) for linoleic acid as mobile phase. The Rf values of oleic acid and linoleic acid were the basis of confirmation of these markers. The total peak areas of standard markers and corresponding formulations were compared and their contents were estimated in all the formulations. The results demonstrated that, analytical method i.e. HPTLC, for marketed formulations concludes that BM, JM demonstrated lowest concentration of Linoleic acid and oleic acid, while laboratory prepared formulations ET, EOT contained more concentration. In conclusion, the analytical method developed herein for standardization of ‘Rasnadi guggul’ preparation including its tablets, will be helpful in obtaining a quality control profile of this formulation.
