Authors : Bhute R.M. , Chandekar C.J.
Page Nos : 26-31
Description :
Bacillus subtilis is isolated from Stale rice. It had aggregation time 52 mins. It showed protease, lipase, and amylase activity. It passes cell surface hydrophobicity 82.8%; resistance to acidic condition (pH 3 for 90 min) and growing in presence of bile salts (in culture medium containing more than 0.15% bile salt). The thermo stable extracellular amylase was isolated and partially purified, the optimum temperature and pH for it was found to be 550C and 6.5 respectively. The maximum amylase production was seen with maltose as carbon source while among the nitrogen sources, complex nitrogen sources support for maximum amylase production.
