Authors : Garode, A. M. , S. M. Waghode
Page Nos : 1-6
Description :
Alkaline α –amylases are used in the starch, textile industries and as an ingredient in detergents. Water samples are collected from Lonar lake of Buldana district (M.S.). The collected water samples are analyzed for isolation of bacteria on the nutrient agar which directly prepared in Lonar lake water. Bacterial isolate was identified on the basis of cultural, morphological and biochemical characterization. The results are found that the Bacillus subtilis produced efficient zone of starch hydrolysis on the starch agar by producing the enzyme alkaline α - amylase. The optimum α - amylase production was studied by using different carbon and nitrogen sources, different pH, incubation temperature and time (hrs). Then assay of the activity of alkaline α amylase performed by DNSA method. The optimum alkaline amylase production was observed in the 1% dextrose and starch as a carbon source and 0.5% peptone and 0.5% meat extracts as a nitrogen source, pH 10.00, incubation temperature 400C and incubation time 72 hrs.
