Authors : Moghe Sandhya, Mangesh Narad, Deepti Laud, Gauri Ade, Ishani Bansod, and Ravindra Moghe
Page Nos : 134-138
Description :
Psoralea corylifolia L. is an important medicinal plant. It is commonly called as Babachi and is an excellent source of psoralene and other major drug compound which are used for the treatment of various deadly diseases such as genital cancer, leucoderma, antidotes effect against poison etc. As the plant is of medicinal value and natural population is drastically reduced, it was investigated for in-vitro regeneration. For regeneration studies of this plant, seeds of good quality was obtained from local nursery and used for in-vitro germination to get various explants. Four different explants such as. Shootip, Cotynodes, Hypocotyl and Leaf explants were selected and they were screened for In-vitro response using Murashige and Skoog modified medium containing different combinations of BAP + KIN + NAA and TDZ. All these growth regulator combinations showed favorable response towards callusing and multiple shoots induction. Result revealed that Leaf explants showed cent percent response against all combinations, while hypocotyl explants sections were less responsive to majority of growth regulators but more favorable to TDZ combinations. The shoot tip and cotynode explants were responded to multiple shoot induction in BAP and NAA combination. The efforts were made to establish regenerated plants after hardening in soil..
Key words: Babachi, psoralea, hypocotyls, shoot tip, cotynode, MS, regeneration, somatic embryogenesis
