Authors : Manisha R. Gejage and R. M. Gejage
Page Nos : 29-31
Description :
Triacylglycerol ester hydrolase activity in haemolymph during larval development of Papilio demoleus (Linnaeus) have been attempted. Larval developmental period of P. demoleus was found to be of 14-days. The larval haemolymph triacylglycerol ester hydrolase noted maximum activity at pH 7.4, incubation time 25 minutes, temperature 370 C, enzyme concentration 1 %, substrate concentration 6 % and Michaelis-Menten constant 0.234×10-2 mM. The triacylglycerol ester hydrolase activity was increased from 6-day to 9-day larval haemolymph and decreased from 9-day to 14-day larval haemolymph of P. demoleus. The maximum triacylglycerol ester hydrolase activity was observed in 9-day larval haemolymph. The triacylglycerol ester hydrolase activity of 9-day larval haemolymph is 1.9477 and 2.2748 fold as compared to 6-day larval haemolymph and 14-day larval haemolymph respectively. Arithmetic mean of 6 to 14-day larval haemolymph triacylglycerol ester hydrolase activity was computed to 0.1998 µ-Equiv. free fatty acids released/100mg /25 minutes. The physiological role of larval liapse in P. demoleus is discussed.
Key words: Triacylglycerol ester hydrolase, haemolymph, larva, insect, P. demoleus (Linn.).
