Authors : Isha Nilkanth Nakshane and Prabhakar R. Bhandari
Page Nos : 25-31
Description :
The present study was aimed to investigate phytochemical constituents and antibacterial activity of aqueous extract of Moringa oleifera leaf belonging to the family Moringaceae on bacterial isolate Staphylococcus aureus. De-ionized distilled water was used to dissolve bioactive compounds of Moringa oleifera leaf for overnight to determined phytochemical constituents. The phytochemical analysis demonstrated the presence of tannins, glycosides, alkaloids, flavonoids and terpenoids are responsible for antibacterial activity. The antibiotic susceptibility test of Staphylococcus aureus was sensitive to Clindamycin and Erythromycin and was found resistance to Penicillin, Oxacillin, Cephalothin and Amoxyclav. The antibacterial activity of aqueous extract of Moringa oleifera against Staphylococcus aureus (gram positive bacteria) using agar well diffusion method in volumes of 300 and 500 µl/well. The results acquired from this study that aqueous extract of Moringa oleifera had antibacterial property against Staphylococcus aureus when obtained from a necessary volume.
