Authors : Rahul L. Meshram and Damini R. Motwani
Page Nos : 251-255
Description :
Amylolytic fungal isolates were obtained by starch-agar plate method from soil sample collected from santra market of Nagpur. Various amylolytic fungi were isolated and out of which three Aspergillus species, named as Aspergillus niger, Aspergillus flavus and Aspergillus oryzae were selected based on clear zone ratio. Amylase production was optimized using basal media. The maximum level of amylase production was achieved from Aspergillus niger, flavus and oryzae after 72 h of cultivation. The optimal temperature for amylase production was in the range of 40 to 45 ° C and pH of the media in the range 7 to 7.5.Under the optimized fermentation conditions Aspergillus species produced almost the similar amount of amylase with organic agro-wastes compared to the basal media. Results reported herein support the notion that all Aspergillus species can be used to produce industrially important amylases by utilizing agro-wastes.
