Authors : Das A. , Thakur K. , Pillai T. and Bramhankar S.
Page Nos : 137-141
Description :
The present investigation shelf life studies of
Bradyrhizobium
by using different carriers Saline
solution, glycerol, carboxymethyl cellulose, soybean oil, paddy bran oil, mineral oil, lignite powder mixed
with 90, 80, 60 and 75 ml
Bradyrhizobium
broth com
bination. Shelf life studies indicated significant
differences in rhizobial colonies. In field experiment, among all the treatments maximum root length were
recorded @ 30 and 45 DAS in T
1
i.e. 10 ml saline solution +90 ml broth (24.77cm/plant, 36.93cm/plan
t)
respectively. Maximum nodulation was recorded by 10 ml saline solution +90 ml
Bradyrhizobium
broth
(13.00). Among 20g CMC + 80 ml
Bradyrhizobium
broth treatment recorded significantly higher number
of nodules (11.36 nodules/ plant) as compared to other
treatment. In paper towel method maximum
germination percentage was recorded in 10ml saline solution + 90 ml
Bradyrhizobium
broth (86%)
followed by 20g CMC + 80 ml
Bradyrhizobium
broth treatment (80.33%). However root and shoot length
was maximum in 10ml s
aline solution + 90 ml
Bradyrhizobium
broth (11.30 cm) and (9.20 cm)
respectively. The maximum seedling vigour index was 1756 by 10ml saline solution +90 ml
Bradyrhizobium
broth seed treatment.
