Authors : Ayodhya Ramulu.Ch , Sammaiah. D, Venkateshwarlu. M, Odelu .G Balaraju.P and Ugandhar. T
Page Nos : 179-184
Description :
In the present investigation, in vitro clonal propagation of seven-month-old Jatropha curcas L. was achieved employing shoot tip explants. Axillary node were cultured on MS medium fortified with Thidiazuron (TDZ) (1.0-3.0 mg/L), IAA (0.5 mg/L)+ TDZ (1.0-3.0 mg/L) for multiple shoot induction. Multiple shoots proliferation was best observed at IAA (0.5 mg/L) + (1.5 mg/L) TDZ from the axillary node explants within four weeks of culture. Shoot number per explants ranged between 2 and 6 Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to NAA (1.0mg/L–3.0mg/L) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 86%. Plants looked healthy with no visually detectable phenotypic variations. The plantlets (12–16- week-old) were successfully acclimatized in soil with 87% survival frequency
Keywords: Axillary Shoot, clonal propagation, Jatropha curcas, shoot tipexplants.
