Authors : M. V. Pachore, B. J. Apparao and A. B. Tambe
Page Nos : 153-156
Description :
An investigation entitled ‘Induced Chlorophyll Mutation in Groundnut (Arachis hypogaea L.)’ was conducted during the kharip (rainy season) 2009 in the experimental field. The germplasm of locally adopted cultivar JL-24 (Phule Pragati) was procured from M.P.K.V.Rahuri, Dist- Ahmednagar (MS), India. Attempts were made to induce genetic variability in yield contributing traits in groundnut (Arachis hypogaea L.) employing gamma radiation to obtain the spectrum and frequency of chlorophyll mutations in M2 generation The seeds of cultivar were presoaked in distilled water for 8 hrs and then treated with four different concentrations of EMS (0.05, 0.10, 0.15, 0.20%) for 04 hrs The uniform and healthy 200 dry seeds were irradiated with five different doses of Gamma rays (100, 150, 200, 250, 300Gy) at Department of Biophysics, Government Institute of Science, Aurangabad (MS), India. Both treated as well as untreated seeds were sown in the experimental field, M2 progeny was raised from the M1 seeds and was screened for different chlorophyll mutations. A variation in frequency of different chlorophyll mutations was observed in different doses. Three different types of chlorophyll mutants, namely, xantha, albino and chlorina, were observed. Out of these mutants, xantha was most frequent while alnina and chlorina were least frequent. The highest frequency of chlorophyll mutations (11.66) was reported in the 100,150 and 250 Gy. There was a dose dependent spectrum and frequency of chlorophyll mutations.
Key words: Gamma rays, mutation, Groundnut
