Authors : Dhulgande G. S. Bagade Manali and Dhasade Puja
Page Nos : 62-65
Description :
Some heavy metals in higher doses may cause metabolic disorders and growth inhibition for most of the plant species. This study was performed in order to evaluate the effect of calcium carbonate (CaCo3) and ferrous sulphate (FeSO4) on seed germination, seedling height and soluble leaf protein content of Moth Bean. The seeds were subjected separately to different concentrations such as 1% and 2% w/v of each chemical solution. It was compared with controlled seeds. Results showed that the germination percentage and rate of germination was significant in all concentrations of both chemical solutions. However, the better germination occurred at 1% concentration of calcium carbonate in petriplate and 2% concentration of ferrous sulphate in soil condition. Also seedling height was calculated. The significant seedling height was recorded at 1% concentrations of each chemical solution in petriplates as well as in soil conditions. Comparatively significant seedling height was recorded in non treated seeds in soil condition. Also among chemical solutions, calcium carbonate showed significant effect on seedling height in petriplate growth while ferrous sulphate showed significant effect on seedling height in soil condition. Soluble protein content was estimated from crude protein extract by Lowry method. The significant soluble protein content was recorded at 0.5 ml crude protein extract of leaves treated by 2% ferrous sulphate chemical concentration. Same results were showed in control at 0.3% ml crude protein extract.
Our results exhibited that the ferrous sulphate had more significant on parameters like seed germination, seedling height and soluble leaf protein content.
Keywords: Moth bean, calcium carbonate, ferrous sulphate, soluble proteins.
