Authors : Kartiki Kawale, S.Chimurkar, A.A. Fulzele
Page Nos : 44-51
Description :
Lipases are widely used enzyme, known for its application in various industries. The fungal strains are
preferred for lipase production since their enzymes are secreted extracellular and the extraction technique
is simple. Thermophilic fungal enzymes are more important because of their stability at high temperature.
During present investigation a thermophilic fungi Rhizomucour pusillus was isolated from decomposing
leaves collected from forest of Nagpur district. Strain improvement of R. pusillus was carried out the
increase Lipase production by using chemical mutagen EMS. Mutant strains derived were evaluated for
yield of lipases production. Lipase activity of mutants was assayed by evaluation of quantity of fatty acid
released in unit time during enzyme reaction and its measured by the quantity of NaOH required to
maintain pH neutral. Present work also includes the effect of natural inducer such as wheat bran and
olive oil on lipase production. It was concluded that the wheat bran is good inducer for lipase production
as compare to olive oil during the process of submerged fermentation
