Authors : B. P. Wadekar and Dharmadhikari S. M.
Page Nos : 01-10
Media composition and growth conditions influence the culture growth of Lactobacillus rhamnosus L43
and the antibacterial performance. The Plackett-Burman design, steepest ascent path design and central
composite design provides an efficient way of a large number of variables and identifying the most
important ones. Different statistical designs prepared and carried step by step, observed and predicted
result using software Minitab 14 and design Expert Version 9. According to PB design and based on low
p value, significant components were found to be lactose, peptone, yeast extract, MgSO4, KH2PO4,
K2HPO4, NaCl had a significant influence (at 95% significant level) on Bacteriocin production. The
optimization medium included lactose of concentration of 20 gm/L, peptone of concentration of 10 gm/L
and MgSO4 of concentration of 0.10gm/L. Predicted value of the diameter of zone of inhibition against
Escherichia coli was 19.66 mm by RSM method and data validation report. The mMRS medium gave the
highest yield of bacteriocin as well as reduced the cost of medium.