Authors : S.B. Patharde and A.M. Chilke
Page Nos : 1-7
Increasing industrialization and anthropogenic activities is the main agent of pollutant discharge into the environment Kidney is an excretory center of the vertebrate body and it carries out excretory function. Alkaline phosphatase is widely studied enzyme and tested for diagnosing the malfunctioning of tissues or organs. The effect of pH, temperature, time and substrate concentration was studied by exposing the enzyme to the range of pH from 8.0 to 11.5, temperature ranging from 300C to 600C, time ranging from 10 to 80 min, and substrate ranging from 0.8 mM to 4.0 mM, however the range of enzyme concentration from 20 µl to 120 µl was used for studying the effect of enzyme concentration on reaction velocity. As negligible work has been done on the kinetics of enzymes in fishes in general and therefore, we aimed to study to find the kinetic properties of this enzyme in the kidney of Clarias gariepinus. Renal alkaline phosphatase exhibited maximum activity at temperature 40°C and pH 9.5 and these are considered to be the optima for this enzyme. However, the enzyme activity was observed to increase consistently with increase in time and enzyme concentrations. Similarly, increase in the concentration of substrate to certain extent found to stabilizes the enzyme activity and it was considered as Vmax. The Vmax and Km values were found to be 196.07 µg/gm wt. of kidney and 5.15 mM from resulted graph.