Issue Description


Authors : Shanti R. Patil, R. D. Deotale, V. Kalamkar, Sneha R. Patil and D. J. Jiotode

Page Nos : 219-222

Description :
Abstract: Experiment was carried out during 2014-15 at Botany Section, College of Agriculture, Nagpur to identify an in vitro protocol for the propagation of white marigold and also to study the effect of gibrellic acid on in vitro propagation. The explants axillary buds were used for culturing in the MS basal fortified with growth regulator like BAP and IAA in different concentrations along with GA3 (14.43µM) and without GA3. GA3 played significant role in the induction of shoot buds as well as suppressing callus formation. The axillary bud explants responded well for all the traits studied. The treatment T16 (MS + BAP 4.44µm + IAA 2.84 µm + GA3 14.43 µm) and T11 (MS + BAP 4.44µm + GA3 14.43 µm) exhibited good performance for response to shoot initiation (%), days to shoot initiation, number of shoots culture-1 and number of shoots elongated culture-1. Average performance recorded for response of shoot initiation(%) was 68.61%, days to shoot initiation was 14.62 days, number of shoots culture-1 was 5.01, number of shoots elongated culture-1 was 3.49, response of root initiation (%) was 57.58 and days to root initiation was 7.81. The shoot propagated from axillary bud were found to root well in treatment T5 (MS+ NAA 0.27µm) and T6 (MS + NAA 0. 54µm). Thus, in vitro propagation of white marigold can be done successfully by inoculating axillary bud explants in T16 (MS + BAP 4.44µm + IAA 2.84 µm + GA3 14.43 µm) and T11 (MS + BAP 4.44µm + GA3 14.43 µm) for shoot induction and proliferation followed by transferring the shoots to T5 (MS+ NAA 0.27µm) for rooting. Key words:- White marigold, shoot tip culture, in vitro propagation

Date of Online: 30 May 2015