Authors : G. Barabde
Page Nos : 209-211
Description :
Abstract:
Chromatographic, Electro chromatographic and different spectroscopic technique play a crucial role in isolating and identifying the potential ingredients in medicinal plants. For chromatographic fingerprinting TLC/HPTLC and HPLC are used for analysis. The optimal separation distance of TLC/HPTLC plates and the use of only one layer volume limit the number of components that can be separated. While in HPLC the sample preparation of medicinal plants before injection of sample is tedious process and also there are chances of losing any component. To overcome limitations of both techniques in case of medicinal plants OPLC is a powerful separation technique that uses a solvent pump into a pressurized ultra micro chamber containing an analytical or preparative planer adsorbent bed to separate components’ of a mixture. OPLC require no or little sample preparation as in TLC and can use the full length of layer for analytical and preparative separation as in HPLC.
Keywords: OPLC, Laminar, Pressurized, ultra micro
