Authors : Ganvir K S
Page Nos : 202-205
Description :
Effect of Gossypol on adrenal- pituitary axis was studied. Gossypol a "China Pill" (C30 H30O8) is phenolic compound isolated from seed of cotton plant (Gossypium species, family Malvaceae). The discovery of this compound by Chinese scientist as male fertility regulating agent (whose action is reversible) is regarded as a breakthrough in the search of male contraceptive. There are also differences of opinion on toxic effects of Gossypol. The clinical trial of this compound in China revealed no side effects. However, side effects such as fatigue, decrease in libido, apetite, dizziness and hypocalcaimic paralysis have been reported by some investigations. In the present investigation attempts have been made to study the histopathological side effects on adrenal-pituitary axis and its mechanism of action.
The experiment was conducted during April and May when the animals (male squirrels) are sexually active. Experimental male animals received Gossypol in two dose, 20 mg/kg B.W./ day and 30 mg/kq. B.W./day for 7 and 5 weeks respectively. At 20 mg. for 7 weeks treatment the cells of zone glomerulosa showed no changes whereas regression and degranulation occurred in 30 mg for 5 weeks treated animals as compared to that of control. In zona fasciculata and zone reticularis Gossypol induced no changes in 20 mg for 7 weeks treated animals, whereas vacuolation and degranulation observed in both of the zones after 30 mg for 5 weeks treatment. Medullary cells show hypertrophy and degranulation in adrenal of both the Gossypol treated groups. ACTH cells of pituitary gland showed no significant changes in 20 mg for 7 weeks treated animals, whereas regressive changes of ACTH Cells were observed in 30 mg for 5 weeks treated animals. Regressive changes in the adrenal cortex appears to be via pituitary gland. Since Gossypol regressed pituitary ACTH cells and deficiency of ACTH causes regression of adrenal cortex. Therefore, it is concluded that regressive changes in the adrenal cortex is via pituitary gland.
