Authors : Shelke Prerna, Malode S. N., & K.N. Sathawane
Page Nos : 39-47
Description :
Brassica (rapeseed–mustard) is the second most important edible oilseed crop in India after groundnut and accounts for nearly 30% of the total oilseeds produced in the country. Brassica campestris L. Yellow seeded variety (BCY-2) was used in present research work. Dry as well as pre-soaked seeds were treated with chemical mutagens Sodium azide (SA) & Ethyl methyl sulphonate (EMS) then sown in experimental field of Department of Botany, GVISH Amravati, to raise M1 generation plants. Selected M1 seeds were used to raise M2 generation. Harvested seeds from M2 generation were used for qualitative analysis of erucic acid by paper chromatography & glucosinolate (GLS) by distrix method. Selected seed samples from all treatments i.e. 18hdry SA; 12h PSW+6h SA & 18h dry EMS; 12hPSW+6hEMS in M2 generation were analyzed further in M3 generation by near infra red spectroscopy (NIRS). Paperchromatography analysis showsmore number plants with low erucic acid level 1.16% in 12hPSW+6h 0.004% EMS. Highest oleic acid 39.26% (0.04%) & 38.48% (0.08%) was recorded in 12hPSW+6hSA & in 18hdry 33.10% (0.006%), 33.03% (0.008%). Higher erucic acid 61.36% was recorded in 18hdry 0.008% SA mutant; whereas, 56.25% erucic acid was recorded in 12hPSW+6h 0.04% EMS. The mutants of M3 showed considerable increase in oleic (18:1) & decrease in linolenic (18:3) as compared to control. Comparative study indicated that PSW/EMS & PSW/SA were effective in inducing (±) & (+) level GSL.Variations in GLS content has been recorded in isolated mutants 18hdry 0.006%SA (109.5µl/g),12hPSW+6h 0.08% SA(108.3µl/g) &12hPSW+6hEMS 0.04% (93.43µmole/g).
