Authors : Manisha Sisodia, Meena Khare, R.R.Kanhere
Page Nos : 10-18
Bisphenol A (BPA) is one of the major industrial worldwide pollutants, and has been extensively detected in surface waters, residues and biota.In the present study, femaleHeteropneustes fossiliswas exposed to various sublethal concentration of BPA and histopathological and biochemical responses were studied. The median lethal concentration of BPA to Heteropneustes fossiliswas calculated by probit analysis method and the value was found as 7.1443 mg/l for 96hrs. Fish were exposed to different sublethal concentrations i.e. to 0.714 mg/L (1/10th), 1.428 mg/L (1/20th) and 2.142 mg/L (1/30th) (Group II, III and IV) using ten specimens in each aquarium for 28 days.At the end of the experiment, fish were dissected and ovary was taken out for histopathological observations. Blood sample was collected by severing the caudal peduncle and processed for the estimation of total plasma protein content, glucose, Aspartate aminotransferase (AST/GOT, EC 126.96.36.199), Alanine transaminase (ALT/GPT, EC 188.8.131.52) and17β-Estradiol. The results shown that BPA was reasonably toxic to the fish Heteropneustes fossilis and toxic effects are depending on concentration dependent. The treatment of sub lethal concentrations of BPA induced substantial alterations in the structural design of ovary. The structural changes mainly include necrosis, increased oocyte atresia, perifollicular cell hyperplasia/hypertrophy, decreased vitellogenesis, and changes in gonadal stagings. BPA treatment showed a significant reduction in plasma protein levels. Exposure to sublethal concentrations of BPA showed a significant and gradual increase in AST (SGOT), ALT (SGPT) and plasma E2 levels all experimental groups. Overall, our study suggests that the incorporation of sublethal concentrations of BPA can alter endocrine system and reproductive function in adult fish and permits further research.