Authors : K. N. Sathawane
Page Nos : 154-159
Description :
Low glucosinolate mustard is one of the major goals for quality oil and meal. To achieve
this goal seeds of Brassica juncea (L.) COSS & CZERN CV. Varuna were treated with different
concentrations of ethyl methane sulfonate and sodium azide. The mutagenized seeds were
sown in the field to raise M1 generation. Seeds of M1 generation were harvested plant – wise
and sown in the field to raise M2 generation. M2 seeds were harvested plant – wise and
analyzed by Tes-tape for glucosinolate content. M2 seeds with Westar level (<20 Amole/gm of
deoiled meal) glucosinolate were selected. Glucosinolate levels were divided into three different
categories depending upon the glucosinolate content. The Westar seeds contain <20 Amole of
glucosinolate while control seeds of Varuna has about >155 Amole of glucosinolate. Hence,
Westar level selected varuna seeds were sown in the field to raise M3 generation. All plants
were selfed and on maturity, harvested plant – wise. Upon screening for Westar level
glucosinolate, the M3 seeds showed variable levels of glucosinolate. M3 seeds were observed to
segregate in medium and high level of glucosinolate. The data obtained indicate positive
possibilities of induced low glucosinolate mutation, however, the following generation need
careful handling, selfing, crossing, screening and large population size.
