Authors : KULSANGE B.K. AND MASRAM S.C.
Page Nos : 72-75
Description :
Teleosts possesses well defined breeding or reproductive cycle. On the basis of shape, size, colour of the ovary different phases viz., preparatory phase, early maturing phase, prespawning phase, spawning phase and spent phase are recognized in teleosts. From the end of preparatory phase to spawning phase developing oocytes passes through different stages. During this journey vitellogenesis and maturation is important event for oocyte development. Various stages of female gametes of Channa punctata were analyzed. Mature Channa punctata were collected by lakes in and around Nagpur city. Different phases of ovarian maturation were confirmed by
calculating gondosomatic index (GSI). Ovarian tissues were fixed in Bouin’s fixative, dehydrated in graded alcohol, cleared in xylene, embedded in paraffin wax and thus blocks were prepared. Blocks were cut in 6-7 µm thick sections. Sections were stained with Haematoxylene and Eosin (HE). Oocytes morphology, nucleus-cytoplasm (N/C) ratio range were studied. Morphometric analysis of different oocytes was carried out with help of Tcapture version 4.3.0.602 software.
Keywords: Channa punctata, oocytes.
