Authors : Y. W. There, V. S. Wadhai and P. Bhandari
Page Nos : 214-218
Description :
Introduction: Staphylococcus aureus is the frequently isolated from patients with serious healthcare-associated infections (HAI). Methicillin & Vancomycin Resistant Staphylococcus aureus infections are a tremendous, and growing, burden for healthcare systems and hospitals, and are associated with significant healthcare costs. So there is need to continuous monitoring of development of resistant to avoid fall in pre antibiotic era. The main objectives of this study was to find out the antibiogram patterns of S.aureus, the prevalence of methicillin resistant S.aureus and demonstration of Vancomycin resistance among MRSA strains at the tribal region Gadchiroli (M.S.), India. Method: From Government district hospitals, Gadchiroli the total 234 clinical samples were collected from different source and 114 samples were positive for S. aureus. Out of these 78 clinical samples were positive for coagulase test. The pure isolates of coagulase positive S.aureus were screened on Oxacillin resistant screen agar and tested for antimicrobial susceptibility by using standard methods. Results: Out of 78 clinical specimens samples were found to be coagulase positive S.aureus. The antibiotics oxacillin, penicillin, erythromycin, gentamycin and tetracyclin had shown maximum resistance on disc diffusion. Amikacin, chloramphenicol and vancomycin antibiotics had showed high sensitivity to all resistant strains of MRSA. The noticeable result in this region was nitrofurantoin had shown around 50% resistance. Of the isolates 50 isolates were methicillin resistant S.aureus (MRSA). According to Disc Diffusion method the Prevalence rate of MRSA was found 64.10 % and Vancomycin resistant among MRSA isolates was found 16%. MIC By Etest method had shown MRSA (48.71%) and VRSA(13.15%). Conclusion: Our study emphasizes the need for continuous monitoring of the antimicrobial susceptibility pattern of S.aureus isolates including MRSA for the selection of appropriate therapy. Gadchiroli is the backward tribal region of Vidarbha, from the present findings it appears that the spread of MRSA in community is very high and the vancomycin which is the last choice for the treatment MRSA strains also shown resistant. So there is need of high alert in hospital settings and need of continuous monitoring and surveillance to control resistance.
Keywords: MRSA, VRSA, Antibiotics and Oxacillin
